Bilirubin Oxidase Antibody, Goat, Polyclonal

Product Overview

• Article Name: Bilirubin Oxidase Antibody, Goat, Polyclonal
• Biozol Catalog Number: ROC-200-101-212-0100
• Supplier Catalog Number: 200-101-212-0100
• Alternative Catalog Number: ROC-200-101-212-0100
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: WB
• Species Reactivity: Fungi
• Immunogen: Bilirubin Oxidase [Myrothecium verrucaria]
• Conjugation: Unconjugated
• Alternative Names: goat anti-Bilirubin Oxidase antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/ml by UV absorbance at 280 nm
• NCBI: 12737
• UniProt: Q12737
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Myrothecium verrucaria
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:5,000 - 1:20,000, IP: 1:100, WB: 1:500 - 1:5,000
• Application Notes: BILIRUBIN OXIDASE Antibody has been tested by western blot and is suitable to be assayed against 1.0 ug of Bilirubin Oxidase [Myrothecium verrucaria] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Goat) code 611-1

Images

Rockland Goat anti Bilirubin Oxidase antibody (200-101-212 lot 6533) was used to detect Bilirubin Oxidase under reducing (R) conditions. A samples of ~1ug of purified protein contained 4% BME and was boiled for 5 minutes and run by SDS-PAGE on a 4-20% acrylamide gel. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (ON 4 C in MB-070). Detection shown was using Dylight 488 conjugated Donkey anti goat (605-741-125 lot 21094 1:10K in TBS/MB-070 1 hr RT) and Dylight 649 conjugated Donkey anti goat (605-743-125 lot 20834 1:10K in TBS/MB-070) 1 hr RT. Images were collected using the BioRad VersaDoc System