Pyranose Oxidase Antibody, Goat, Polyclonal

Product Overview

• Article Name: Pyranose Oxidase Antibody, Goat, Polyclonal
• Biozol Catalog Number: ROC-200-101-235S
• Supplier Catalog Number: 200-101-235S
• Alternative Catalog Number: ROC-200-101-235S
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: WB
• Species Reactivity: Bacteria
• Immunogen: Pyranose Oxidase [E.coli]
• Conjugation: Unconjugated
• Alternative Names: goat anti-Pyranose Oxidase Antibody, P2Ox, Pyranose oxidase, PROD, POD, POx, Pyranose:oxygen 2-oxidoreductase, Glucose 2-oxidase, FAD-oxidoreductase

Product Properties

• Clonality: Polyclonal
• NCBI: 5
• UniProt: P79076
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Escherichia coli
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:5,000 - 1:20,000, IP: 1:100, WB: 1:500 - 1:5,000
• Application Notes: Pyranose Oxidase Antibody has been tested by western blot and is suitable to be assayed against 1.0 µg of Pyranose Oxidase [E.coli] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) code 605-4302 and (ABTS (2

Images

Rockland Goat anti Pyranose Oxidase antibody (200-101-235 lot 8178) was used to detect pyranose oxidase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (ON 4 C in MB-070). Detection shown was using Dylight 488 conjugated Donkey anti goat (605-741-125 lot 21094 1:10K in TBS/MB-070 1 hr RT) . Images were collected using the BioRad VersaDoc System