Uricase Antibody Peroxidase Conjugated, HRP, Goat, Polyclonal

Product Overview

• Article Name: Uricase Antibody Peroxidase Conjugated, HRP, Goat, Polyclonal
• Biozol Catalog Number: ROC-200-103-092S
• Supplier Catalog Number: 200-103-092S
• Alternative Catalog Number: ROC-200-103-092S
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Bacteria
• Immunogen: Anti-uricase Antibody was produced by repeated immunizations with bacillus uricase protein.
• Conjugation: HRP
• Alternative Names: goat anti-uricase antibody, HRP conjugated goat anti-uricase antibody, Urate oxidase antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• NCBI: 3202700
• UniProt: Q5WBJ3
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Bacillus
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:1,000 - 1:2,500, IP: 1:100, WB: 1:500 - 1:1,000
• Application Notes: Anti-Uricase antibody has been tested by ELISA and western blot and is suitable for IP. Researchers should determine optimal titers for applications that are not stated below.

Images

Western Blot of Goat anti-Uricase Peroxidase Conjugated antibody. Lane 1: purified Uricase under reducing conditions. Lane 2: purified Uricase under reducing conditions. Lane 3: purified Uricase under non-reducing conditions. Lane 4: purified Uricase under non-reducing conditions Load: ~1.0 µg (Lane 1 and 3) and 0.25 µg (Lane 2 and 4). Primary antibody: Uricase Peroxidase Conjugated Antibody at 1:5,000 overnight at 4C. Secondary antibody: DyLight(TM) 649 goat secondary antibody at 1:10,000 for 90 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 58 kDa, 58 kDa for Uricase. Other band(s): Uricase splice variants and isoforms.