Anti-Plasminogen has been tested by ELISA and western blot. This product is assayed against 1.0 µg of Plasminogen [Human Plasma] in a standard capture ELISA using ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code ABTS-100 as a substrate
Rockland Goat anti Plasminogen antibody (200-101-208 lot 6571) was used to detect Plasminogen under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (ON 4 C in MB-070). Detection shown was using Dylight 649 conjugated Donkey anti goat (605-743-125 lot 20834 1:10K in TBS/MB-070) 1 hr RT. Images were collected using the BioRad VersaDoc System.
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