Glutamine Synthetase Antibody Peroxidase Conjugated, HRP, Goat, Polyclonal

Product Overview

• Article Name: Glutamine Synthetase Antibody Peroxidase Conjugated, HRP, Goat, Polyclonal
• Biozol Catalog Number: ROC-200-103-238-0100
• Supplier Catalog Number: 200-103-238-0100
• Alternative Catalog Number: ROC-200-103-238-0100
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Species Reactivity: Bacteria
• Immunogen: Glutamine Synthetase [native from brevibacterium species]
• Conjugation: HRP
• Alternative Names: goat anti-Glutamine Synthetase Antibody HRP Conjugation, Peroxidase Conjugated goat anti-Glutamine Synthetase Antibody, Glutamine synthetase

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• NCBI: 3345165
• UniProt: Q79VE3
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Microbial
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:2,000 - 1:10,000, IP: 1:100, WB: 1:500 - 1:2,500
• Application Notes: Anti-Glutamine Synthetase has been assayed against 1.0 ug of Glutamine Synthetase [Microbial] in a standard capture ELISA using ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code ABTS-100 as a substrate for 30 minutes at room temperature

Images

Rockland Goat anti Glutamine Synthetase antibody (200-101-238 lot 8182) was used to detect Glutamine Synthetase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (ON 4 C in MB-070). Detection shown was using Dylight 649 conjugated Donkey anti goat (605-743-125 lot 20834 1:10K in TBS/MB-070) 1 hr RT. Images were collected using the BioRad VersaDoc System.