Protein A Antibody Biotin Conjugated, Goat, Polyclonal

Product Overview

• Article Name: Protein A Antibody Biotin Conjugated, Goat, Polyclonal
• Biozol Catalog Number: ROC-200-106-077S
• Supplier Catalog Number: 200-106-077S
• Alternative Catalog Number: ROC-200-106-077S
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: DOT, WB
• Immunogen: Protein A [Staphylococcus aureus]
• Conjugation: Biotin
• Alternative Names: goat anti-Protein A Antibody, Biotin Conjugated goat anti-Protein A Antibody, Protein A Biotin, Protein A BAC Antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:2,000-1:10,000, IHC: 1:500-1:2,500, WB: 1:1,000-1:5,000
• Application Notes: Anti-Protein A Biotin Conjugated has been tested by dot blot and western blot. This product is suitable to be assayed against 1.0 ug of Protein A in a standard capture ELISA using Peroxidase Conjugated Streptavidin S000-03 and ABTS (2,2-azino-bis-[3-et

Images

Western Blot of Goat anti-Protein A Antibody. Lane 1: Protein A Reduced. Lane 2: Protein A Reduced. Lane 3: Protein A Non-Reduced. Lane 4: Protein A Non-Reduced. Load: Lane 1 and 3 - 1.0 µg per lane, Lane 2 and 4 - 0.25 ug per lane. Primary antibody: Protein A antibody at 1:5,000 for overnight at 4C. Secondary antibody: DyLight(TM) 649 goat secondary antibody at 1:10,000 for 90 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~50 kDa for Protein A. Other band(s): Protein A splice variants and isoforms.