Cholesterol Oxidase Antibody Biotin Conjugated, Goat, Polyclonal

Product Overview

• Article Name: Cholesterol Oxidase Antibody Biotin Conjugated, Goat, Polyclonal
• Biozol Catalog Number: ROC-200-106-213S
• Supplier Catalog Number: 200-106-213S
• Alternative Catalog Number: ROC-200-106-213S
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: DOT, ELISA, WB
• Species Reactivity: Bacteria
• Immunogen: Cholesterol Oxidase [Streptomyces]
• Conjugation: Biotin
• Alternative Names: goat anti-Cholesterol Oxidase Antibody biotin Conjugation, biotin Conjugated goat anti-Cholesterol Oxidase Antibody, choA antibody, choD antibody, choM antibody, choS antibody, COD antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/ml by UV absorbance at 280 nm
• NCBI: 12676
• UniProt: P12676
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Streptomyces
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:5,000 - 1:20,000, IP: 1:100, WB: 1:500 - 1:5,000
• Application Notes: Anti-Cholesterol Oxidase has been tested by ELISA, dot blot, and western blot. This product is assayed against 1.0 ug of Cholesterol Oxidase in a standard capture ELISA using Peroxidase Conjugated Streptavidin S000-03 and ABTS (2,2-azino-bis-[3-ethylbe

Images

Rockland Goat anti Cholesterol oxidase antibody (200-101-213 lot 6534) was used to detect purified cholesterol oxidase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified cholesterol oxidase contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with a 1:3000 dilution of primary antibody (ON 4 C in MB-070). Detection shown was using Dylight 488 conjugated Donkey anti goat (605-741-125 lot 21094 1:10K in TBS/MB-070 1 hr RT). Images were collected using the BioRad VersaDoc System.