0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Bacillus
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:5,000 - 1:25,000, WB: 1:500 - 1:3,000
Application Notes:
Anti-Glucose Dehydrogenase has been assayed against 1.0 ug of Glucose Dehydrogenase in a standard capture ELISA using Peroxidase Conjugated Streptavidin S000-03 and ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code ABTS-100 as a substr
Rockland Goat anti Glucose Dehydrogenase antibody (100-101-214 lot 6454) was used to detect purified Glucose Dehydrogenase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (ON 4 C in MB-070). Detection shown was using Dylight 488 conjugated Donkey anti goat (605-741-125 lot 21094 1:10K in TBS/MB-070 1 hr RT). Images were collected using the BioRad VersaDoc System
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