N-Acylmannosamine-1-Dehydrogenase Antibody Biotin Conjugated, Goat, Polyclonal

Product Overview

• Article Name: N-Acylmannosamine-1-Dehydrogenase Antibody Biotin Conjugated, Goat, Polyclonal
• Biozol Catalog Number: ROC-200-106-228-0100
• Supplier Catalog Number: 200-106-228-0100
• Alternative Catalog Number: ROC-200-106-228-0100
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: WB
• Species Reactivity: Bacteria
• Immunogen: N-Acyl Mannosamine-1-Dehydrogenase [Recombinant Sequence from Pseudomonas expressed in E.coli ]
• Conjugation: Biotin
• Alternative Names: goat anti-N-Acylmannosamine-1-Dehydrogenase Antibody biotin Conjugation, Biotin Conjugated goat anti-N-Acylmannosamine-1-Dehydrogenase Antibody, N-acylmannosamine 1-dehydrogenase, NAM-DH

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• NCBI: 7614553
• UniProt: P22441
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Pseudomonas
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:1,000 - 1:5,000, IP: 1:100, WB: 1:250 - 1:1,000
• Application Notes: Anti-N-Acylmannosamine-1-Dehydrogenase antibody has been tested by western blot and is suitable to be assayed against 1.0 µg of N-Acylmannoseamine-1-Dehydrogenase in a standard capture ELISA using Peroxidase Conjugated Streptavidin S000-03 and ABTS (2,2

Images

Rockland Goat anti N-acylmanosamino-1-Dehydrogenase antibody (200-101-228 lot 8181) was used to detect purified N-acylmanosamino-1-Dehydrogenase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (ON 4 C in MB-070). Detection shown was using Dylight 488 conjugated Donkey anti goat (605-741-125 lot 21094 1:10K in TBS/MB-070 1 hr RT). Images were collected using the BioRad VersaDoc System.

Western Blot of Goat anti-N-Acylmannoseamide 1-Dehydrogenase Antibody Biotin Conjugated. Lane 1: N-Acylmannoseamide 1-Dehydrogenase. Load: 50 ng per lane. Primary antibody: Goat anti-N-Acylmannoseamide 1-Dehydrogenase Antibody Biotin Conjugated 1:1,000 overnight at 4C. Secondary antibody: HRP Streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 27.5 kDa, observed at 30 kDa for N-Acylmannoseamide 1-Dehydrogenase.