Glucoamylase Antibody Biotin Conjugated, Goat, Polyclonal

Product Overview

• Article Name: Glucoamylase Antibody Biotin Conjugated, Goat, Polyclonal
• Biozol Catalog Number: ROC-200-106-234S
• Supplier Catalog Number: 200-106-234S
• Alternative Catalog Number: ROC-200-106-234S
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Other
• Immunogen: Glucoamylase [Rhizopus]
• Conjugation: Biotin
• Alternative Names: goat anti-Glucoamylase Antibody biotin Conjugation, biotin Conjugated goat anti-Glucoamylase Antibody, 1 4 alpha D glucan glucohydrolase antibody, Amyloglucosidase antibody, Gluc 1 antibody, Gluc 2 antibody, Gluc 3 antibody, Glucan 1 4 alpha glucosidase antibody, Glucan 14 alpha glucosidase antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• NCBI: 07683
• UniProt: P07683
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Rhizopus
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:2,000 - 1:10,000, IP: 1:100, WB: 1:500 - 1:2,500
• Application Notes: Anti-Glucoamylase biotin has been tested by ELISA and western blot. This product is assayed against 1.0 µg of Glucoamylase in a standard capture ELISA using Peroxidase Conjugated Streptavidin S000-03 and ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulf

Images

Rockland Goat anti Glucoamylase antibody (100-101-234 lot 7844) was used to detect purified glucoamylase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (ON 4 C in MB-070). Detection shown was using Dylight 488 conjugated Donkey anti goat (605-741-125 lot 21094 1:10K in TBS/MB-070 1 hr RT) . Images were collected using the BioRad VersaDoc System