Beta-Phosphoglucomutase Antibody Biotin Conjugated, Goat, Polyclonal

Product Overview

• Article Name: Beta-Phosphoglucomutase Antibody Biotin Conjugated, Goat, Polyclonal
• Biozol Catalog Number: ROC-200-106-237S
• Supplier Catalog Number: 200-106-237S
• Alternative Catalog Number: ROC-200-106-237S
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Bacteria
• Immunogen: beta Phosphoglucomutase [Lactococcus lacti]
• Conjugation: Biotin
• Alternative Names: goat anti-Beta-Phosphoglucomutase Antibody biotin Conjugation, biotin conjugated goat anti-Beta PGM antibody, Glucose phosphomutase 1 antibody, Glucose phosphomutase antibody, PGM antibody, pgmB antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• NCBI: 1114041
• UniProt: P71447
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Lactococcus lacti
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB: 1:200 - 1:1,000
• Application Notes: Anti-Beta-Phosphoglucomutase Biotin has been tested by ELISA and western blot. This product is assayed against 1.0 ug of Beta-Phosphoglucomutase in a standard capture ELISA using Peroxidase Conjugated Streptavidin S000-03 and ABTS (2,2-azino-bis-[3-eth

Images

Rockland Goat anti antibody (200-101-237 lot 8180) was used to detect purified Beta Phospho Glucomutase under reducing (R) and non-reducing (NR) conditions. Reduced samples of protein contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (ON 4 C in MB-070). Detection shown was using Dylight 488 conjugated Donkey anti goat (605-741-125 lot 21094 1:10K in TBS/MB-070 1 hr RT). Images were collected using the BioRad VersaDoc System.