Glutamine Synthetase Antibody Biotin Conjugated, Goat, Polyclonal

Product Overview

• Article Name: Glutamine Synthetase Antibody Biotin Conjugated, Goat, Polyclonal
• Biozol Catalog Number: ROC-200-106-238S
• Supplier Catalog Number: 200-106-238S
• Alternative Catalog Number: ROC-200-106-238S
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Species Reactivity: Bacteria
• Immunogen: Glutamine Synthetase [Microbial]
• Conjugation: Biotin
• Alternative Names: goat anti-Glutamine Synthetase Antibody biotin Conjugation, biotin Conjugated goat anti-Glutamine Synthetase Antibody, Glutamine synthetase

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• NCBI: 3345165
• UniProt: Q79VE3
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Microbial
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:2,000 - 1:10,000, IP: 1:100, WB: 1:500 - 1:2,500
• Application Notes: Anti-Glutamine Synthetase (microbial) antibody has been assayed against 1.0 µg of Glutamine Synthetase in a standard capture ELISA using Peroxidase Conjugated Streptavidin S000-03 and ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code A

Images

Rockland Goat anti Glutamine Synthetase antibody (200-101-238 lot 8182) was used to detect Glutamine Synthetase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (ON 4 C in MB-070). Detection shown was using Dylight 649 conjugated Donkey anti goat (605-743-125 lot 20834 1:10K in TBS/MB-070) 1 hr RT. Images were collected using the BioRad VersaDoc System.