Fibrinogen Antibody Biotin Conjugated, Goat, Polyclonal

Product Overview

• Article Name: Fibrinogen Antibody Biotin Conjugated, Goat, Polyclonal
• Biozol Catalog Number: ROC-200-106-240-0100
• Supplier Catalog Number: 200-106-240-0100
• Alternative Catalog Number: ROC-200-106-240-0100
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: ELISA, IHC, WB
• Species Reactivity: Human
• Immunogen: Fibrinogen [Human Plasma]
• Conjugation: Biotin
• Alternative Names: goat anti-Fibrinogen Antibody biotin Conjugation, biotin Conjugated goat anti-Fibrinogen Antibody, FGA antibody, FGA protein antibody, FGB antibody, FGG antibody, Fib2 antibody, Fibrinogen A alpha polypeptide antibody, Fibrinogen A alpha polypeptide chain antibody, Fibrinogen alpha chain antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• NCBI: 2243
• UniProt: P02671
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Human
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:2,000 - 1:10,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
• Application Notes: Anti-Fibrinogen Biotin antibody has been tested by ELISA, western blot, and immunohistochemistry. This product is assayed against 1.0 µg of Fibrinogen in a standard capture ELISA using Peroxidase Conjugated Streptavidin S000-03 and ABTS (2,2-azino-bis-

Images

Western Blot of Goat anti-Fibrinogen antibody. Lane 1: Fibrinogen under reducing conditions. Lane 2: none. Load: 1 µg per lane. Primary antibody: Fibrinogen antibody at 1:3000 for overnight at 4C. Secondary antibody: Dylight 488 conjugated Donkey anti goat secondary antibody at 1:10,000 for 45 min at RT. Block: TBS/MB-070 1 hr RT.

Western Blot of Goat anti-Fibrinogen Antibody (Human Plasma) Biotin Conjugated. Lane 1: Fibrinogen (Human Plasma). Load: 50 ng per lane. Primary antibody: Goat anti-Fibrinogen Antibody (Human Plasma) Biotin Conjugated at 1:1,000 overnight at 4C. Secondary antibody: HRP streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 minutes at RT. Predicted/Observed size: 56 kDa, 56 kDa for Fibrinogen ß-chain.

Immunohistochemistry of Goat Anti-Fibrinogen antibody. Tissue: human liver tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Fibrinogen antibody at 1:500 for 1 h at RT. Secondary antibody: Peroxidase goat secondary antibody at 1:10,000 for 45 min at RT. Localization: Fibrinogen is localized in plasma. Staining: Fibrinogen as precipitated red signal with hematoxylin purple nuclear counterstain.