Papain Antibody, Goat, Polyclonal

Product Overview

• Article Name: Papain Antibody, Goat, Polyclonal
• Biozol Catalog Number: ROC-200-1174S
• Supplier Catalog Number: 200-1174S
• Alternative Catalog Number: ROC-200-1174S
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: WB
• Species Reactivity: Plant
• Immunogen: Anti-Papain Antibody was produced by repeated immunizations with pork Papain protein isolated from papaya.
• Conjugation: Unconjugated
• Alternative Names: goat anti-Papain Antibody, Allergen Car p 1 antibody, Papaya proteinase I antibody, PPI antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/ml by UV absorbance at 280 nm
• NCBI: 00784
• UniProt: P00784
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Carica papaya
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:3,000, WB: 1:1,000 - 1:5,000
• Application Notes: Anti-Papain antibody has been tested by western blotting to detect a single band of the expected apparent molecular weight and for ELISA. Researchers should determine optimal titers for applications that are not stated below.

Images

Western Blot of Goat anti-Papain Antibody. Lane 1: Papain. Load: 50 ng per lane. Primary antibody: Goat anti-Papain Antibody at 1:1,000 overnight at 4C. Secondary antibody: HRP goat secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 23 kDa, 23 kDa for Papain.