Protein A Antibody Peroxidase Conjugated, HRP, Goat, Polyclonal Preis auf Anfrage

Product Overview

• Article Name: Protein A Antibody Peroxidase Conjugated, HRP, Goat, Polyclonal Preis auf Anfrage
• Biozol Catalog Number: ROC-200-1377
• Supplier Catalog Number: 200-1377
• Alternative Catalog Number: ROC-200-1377
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Immunogen: Protein A [Staphylococcus aureus]
• Conjugation: HRP
• Alternative Names: goat anti-Protein A Antibody, HRP Conjugated goat anti-Protein A Antibody, Protein A Peroxidase, Protein A HRP Antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 10.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:10,000-1:50,000, IHC: 1:500-1:2,500, WB: 1:1,000-1:5,000
• Application Notes: This product has been assayed against 1.0 ug of Protein A [Staphylococcus aureus] in a standard capture ELISA using ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code ABTS-100 as a substrate for 30 minutes at room temperature. A working

Images

Western Blot of Goat anti-Protein A Antibody. Lane 1: Protein A Reduced. Lane 2: Protein A Reduced. Lane 3: Protein A Non-Reduced. Lane 4: Protein A Non-Reduced. Load: Lane 1 and 3 - 1.0 µg per lane, Lane 2 and 4 - 0.25 ug per lane. Primary antibody: Protein A antibody at 1:5,000 for overnight at 4C. Secondary antibody: DyLight(TM) 649 goat secondary antibody at 1:10,000 for 90 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~50 kDa for Protein A. Other band(s): Protein A splice variants and isoforms.