0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:2,000 - 1:10,000, WB: 1:500 - 1:2,000
Application Notes:
Anti-Carbonic anhydrase I Biotin Antibody has been tested by ELISA and western blot. This product is assayed against 1.0 ug of Carbonic Anhydrase I in a standard capture ELISA using Peroxidase Conjugated Streptavidin S000-03 and ABTS (2,2-azino-bis-[3-
ELISA Results of Goat Anti-Carbonic Anhydrase I Antibody Biotin Conjugated tested against purified Carbonic Anhydrase I Biotin. Each well was coated in duplicate with 1.0 µg of Carbonic Anhydrase I. The working dilution is 1:6900. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer (p/n MB-076), Streptavidin-HRP conjugated (p/n S000-03), and TMB substrate (p/n TMBE-1000).
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