Anti-Papain Biotin antibody has been tested by ELISA and western blot. Expect band at 38.9 kDa in appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
Western Blot of Goat anti-Papain Antibody Biotin Conjugated. Lane 1: Papain. Load: 50 ng per lane. Primary antibody: Goat anti-Papain Antibody Biotin Conjugated at 1:1,000 overnight at 4C. Secondary antibody: HRP Streptavidin (p/n S000-03) secondary antibody at 1:40,000 for 30 minutes at RT. Block: MB-070 for 30 minutes at RT. Predicted/Observed size: 23 kDa, 23 kDa for Papain.
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