This HSP27 monoclonal antibody was produced by repeated immunizations with a prokaryotic recombinant protein corresponding to the full length human hsp27 protein.
Conjugation:
Unconjugated
Alternative Names:
mouse anti-HSP27 antibody, mouse anti-Heat shock protein beta-1 antibody, HspB1 antibody, Heat shock 27 kDa protein antibody, Stress-responsive protein 27 antibody, SRP27 antibody, Estrogen-regulated 24 kDa protein antibody, 28 kDa heat shock protein antibody
0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:10,000 - 1:50,000, WB: 1:500 - 1:1,000
Application Notes:
This protein A purified monoclonal antibody against human Hsp27 has been tested for use in immunoblotting and ELISA and is suitable in immunoprecipitation, immunohistochemistry, and immunocytochemistry. The antibody recognizes a 27 kDa band corresponding
Mab anti-Human HSP27 antibody (clone G3.1) is shown to detect human HSP27 by western blot. Detection occurs after 10 µg of a HeLa whole cell lysate is loaded per lane. The blot was incubated with a 1:1,000 dilution of Mab anti-Human HSP27 at room temperature for 30 min followed by detection using IRDye(TM)800 labeled Goat-a-Mouse IgG [H&L] (610-132-121) diluted 1:5,000. A single band corresponding to human HSP27 is detected at ~27 kDa when compared with known molecular weight standards (not shown). The antibody may be used to detect endogenous human HSP27. IRDye(TM)800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
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