KLH Antibody, IgG1, Clone: [12B4.G3.A8], Mouse, Monoclonal

Product Overview

• Article Name: KLH Antibody, IgG1, Clone: [12B4.G3.A8], Mouse, Monoclonal
• Biozol Catalog Number: ROC-200-301-466
• Supplier Catalog Number: 200-301-466
• Alternative Catalog Number: ROC-200-301-466
• Manufacturer: Rockland Immunochemicals
• Host: Mouse
• Category: Antikörper
• Application: ELISA
• Species Reactivity: Mollusc
• Immunogen: Anti-KLH (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with KLH (Keyhole Limpet Hemocyanin) followed by hybridoma development.
• Conjugation: Unconjugated
• Alternative Names: mouse anti-KLH Antibody, Keyhole Limpet Hemocyanin, KLH antibody

Product Properties

• Clonality: Monoclonal
• Concentration: 1.004 mg/mL by UV absorbance at 280 nm
• Clone Designation: [12B4.G3.A8]
• Isotype: IgG1
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Application Notes: Anti-KLH has been tested by ELISA. It can be used as a control in various assays and in ELISA assays to detect KLH or KLH conjugates. Specific conditions for reactivity should be optimized by the end user.

Images

ELISA Results of Mouse Anti-KLH Antibody tested against purified KLH protein. Each well was coated in duplicate with 1.0 µg of KLH. The working dilution is 1:170,000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugated Stabilizer (p/n MB-076), Rabbit Anti-Mouse IgG HRP conjugated (p/n 610-403-C46) and TMB substrate (p/n TMBE-1000).