Anti-STAT5 phospho Y694 monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Y694 of mouse STAT5a protein.
Conjugation:
Unconjugated
Alternative Names:
mouse anti-STAT5 pY694 Antibody, MGF antibody, Signal Transducer and Activator of Transcription 5A antibody, STAT 5 antibody
Phospho STAT5 pY694 monoclonal antibody has been tested by ELISA, western blot, immunohistochemistry, and immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 91 kDa in size corresponding
Immunofluorescent STED Microscopy of Anti-Stat5 pY694 (MOUSE) Monoclonal Antibody in 3T3 cells. Anti-Stat5 pY694 at 1:500 detecting Stat5 in 3T3 cells. Staining: Stat5 pY694 protein [Red]. Tubulin [Green].
Western blot using Rocklands Protein A purified Mouse Monoclonal anti-Stat5 pY694 antibody shows detection of phosphorylated Stat5 (indicated by arrowhead at ~91 kDa) in NK92 cells after 30 min treatment with 1Ku of IL-2 (lane 2). No reactivity is seen for non-phosphorylated Stat5 in untreated cells (lane 1). The membrane was probed with the primary antibody at a 1:1,000 dilution, overnight at 4 C. For detection DyLight(TM)800 conjugated Gt-a-Mouse IgG (p/n 610-145-002) was used at a 1:20,000 dilution for 30 min at room temperature followed by visualization using a VersaDoc(TM) MP 4000 imaging system (Bio-Rad).
Immunohistochemistry of Monoclonal Anti-Stat5 pY694 Antibody. Tissue: human breast tissue (40X). Fixation: formalin fixed paraffin embedded (FFPE). Antigen retrieval: steam sections in 0.1 M sodium citrate buffer, pH 6, for 20 min. Rinse with 1XTBST. Primary antibody: Anti-Stat5pY694 at 20 µg/mL. Localization: breast epithelium with moderate nuclear staining. Staining: Stat5 pY694 as precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal communication, Andrew Elston, Lifespan Biosciences, Seattle, WA.
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