Anti-Mouse monoclonal CD4 antibody was produced by repeated immunizations in mice with a 193 amino acid recombinant protein from near the N-terminus of human CD4.
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:500 - 1:10,000, IHC: 5µg/mL, IF Microscopy: User Optimized, WB: 0.5-1 µg/mL
Application Notes:
Anti-CD4 Antibody has been tested for use in ELISA, immunohistochemistry, Immunofluorescence, and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 51 kDa in Western Blots of specific
Western Blot of Mouse anti-CD4 antibody. Lane A: rat lung tissue lysate at 0.5 µg/mL. Lane B: rat lung tissue lysate at 1 µg/mL. Primary antibody: CD4 antibody overnight at 4°C. Secondary antibody: Mouse HRP secondary antibody. Block: 5% BLOTTO. Predicted/Observed size: 51 kDa, 49 kDa for CD4.
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