CD4 Antibody [9H5A8], IgG1, Mouse, Monoclonal

Product Overview

• Article Name: CD4 Antibody [9H5A8], IgG1, Mouse, Monoclonal
• Biozol Catalog Number: ROC-200-301-AJ0
• Supplier Catalog Number: 200-301-AJ0
• Alternative Catalog Number: ROC-200-301-AJ0
• Manufacturer: Rockland Immunochemicals
• Host: Mouse
• Category: Antikörper
• Application: ELISA, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Anti-Mouse monoclonal CD4 antibody was produced by repeated immunizations in mice with a 193 amino acid recombinant protein from near the N-terminus of human CD4.
• Conjugation: Unconjugated
• Alternative Names: CD4 Antibody [9H5A8] , CD4mut

Product Properties

• Clonality: Monoclonal
• Concentration: 1 mg/mL by UV absorbance at 280 nm
• Clone Designation: [9H5A8]
• Isotype: IgG1
• NCBI: 920
• UniProt: P01730
• Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Human
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:500 - 1:10,000, IHC: 5µg/mL, WB: 0.5-4µg/mL
• Application Notes: Anti-CD4 Antibody has been tested for use in ELISA, immunohistochemistry, and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 51 kDa in Western Blots of specific cell lysates and ti

Images

Western Blot of Mouse anti-CD4 antibody. Lane A: human thymus tissue lysate at 0.5 µg/mL. Lane B: human thymus tissue lysate at 1 µg/mL. Primary antibody: CD4antibody overnight at 4°C. Secondary antibody: Mouse HRP secondary antibody. Block: 5% BLOTTO. Predicted/Observed size: 51 kDa, 49 kDa for CD4.