0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:50,000 - 1:100,000, WB: 2.5-5 µg/mL
Application Notes:
Anti-EndoG Antibody has been tested for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 33 kDa in Western Blots of specific cell lysates and tissues. Validated in h
Western Blot of Mouse anti-EndoG antibody. Lane A: HepG2 cell lysate at 2.5 µg/mL. Lane B: HepG2 cell lysate at 5 µg/mL. Primary antibody: EndoG antibody overnight at 4°C. Secondary antibody: Mouse HRP secondary antibody. Block: 5% BLOTTO. Predicted/Observed size: 32 kDa, 34 kDa for EndoG.
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