0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:10,000, WB: 1 µg/mL
Application Notes:
Anti-KLF4 Antibody has been tested for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 55 kDa in Western Blots of specific cell lysates and tissues. Validated in mo
Western Blot of Mouse anti-KLF4 antibody. Lane A: mouse liver tissue lysate in the absence of blocking peptide. Lane B: mouse liver tissue lysate in the presence of blocking peptide. Primary antibody: KLF4 antibody at 1 µg/mL overnight at 4°C. Secondary antibody: Mouse HRP secondary antibody. Block: 5% BLOTTO. Predicted/Observed size: 51 kDa, 44 kDa for KLF4.
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