COBRA 1 Antibody, IgG1, Clone: [F7E4], Mouse, Monoclonal

Catalog Number: ROC-200-301-D18
Article Name: COBRA 1 Antibody, IgG1, Clone: [F7E4], Mouse, Monoclonal
Biozol Catalog Number: ROC-200-301-D18
Supplier Catalog Number: 200-301-D18
Alternative Catalog Number: ROC-200-301-D18
Manufacturer: Rockland Immunochemicals
Host: Mouse
Category: Antikörper
Application: WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Anti-COBRA1 Monoclonal Antibody was produced in mouse by repeated immunizations with recombinant full length human COBRA1 purified from E.coli.
Conjugation: Unconjugated
Alternative Names: COBRA1, Negative elongation factor B, NELF-B, Cofactor of BRCA1
Clonality: Monoclonal
Concentration: Titrated value sufficient to run approximately 10 mini blots.
Clone Designation: [F7E4]
Isotype: IgG1
NCBI: 25920
UniProt: Q8WX92
Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
Form: Liquid
Target: Human
Antibody Type: Primary Antibody
Application Dilute: ELISA: 1:10,000, WB: 1:1000
Application Notes: Anti-COBRA 1 antibody has been tested by ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 62 kDa in size corresponding to COBRA1 proteins in neonatal rat brain lysate or ot
Western Blot of Rabbit Anti-COBRA1 Antibody. Lane 1: neonatal rat brain lysate. Lane 2: none. Load: 10 µg per lane. Primary antibody: COBRA1 antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800(TM) mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~ 62kDa/~62kDa for COBRA1 protein. Other band(s): none.