Anti-PUMA antibody was produced In mice by repeated immunizations with a recombinant protein corresponding to amino acids 76 - 170 of human PUMA-alpha.
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:20,000 - 1:50,000, WB: 2.5-5 µg/mL
Application Notes:
Anti-PUMA Antibody has been tested for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 21 kDa in Western Blots of specific cell lysates and tissues. Validated in hu
Western Blot of Mouse anti-PUMA antibody. Lane A: K562 cell lysate at 2.5 µg/mL. Lane B: K562 cell lysate at 5 µg/mL. Primary antibody: PUMA antibody overnight at 4°C. Secondary antibody: Mouse HRP secondary antibody. Block: 5% BLOTTO. Predicted/Observed size: 20 kDa with 3 isoforms, 27 kDa for PUMA.
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