Alpha A Crystallin Antibody, IgG1, Clone: [1H3.B8], Mouse, Monoclonal

Product Overview

• Article Name: Alpha A Crystallin Antibody, IgG1, Clone: [1H3.B8], Mouse, Monoclonal
• Biozol Catalog Number: ROC-200-301-E84
• Supplier Catalog Number: 200-301-E84
• Alternative Catalog Number: ROC-200-301-E84
• Manufacturer: Rockland Immunochemicals
• Host: Mouse
• Category: Antikörper
• Application: IF, WB
• Species Reactivity: Bovine, Human, Mouse, Rat
• Immunogen: Alpha Crystallin Antibody was produced in mice by repeated immunizations with Native Alpha Crystallin protein.
• Conjugation: Unconjugated
• Alternative Names: Heat shock protein beta4, Acry 1, CRYA1, CRYAA, HspB4, Alpha-crystallin A chain

Product Properties

• Clonality: Monoclonal
• Concentration: 1.0 mg/ml by UV absorbance at 280 nm
• Clone Designation: [1H3.B8]
• Isotype: IgG1
• NCBI: 1409
• UniProt: P02489
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Human
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:200, WB: 1:2000
• Application Notes: Anti-Alpha A Crystallin Antibody has been tested in WB and IF and is recommended for use in ELISA. Expect a band approximately ~20kDa protein corresponding to the molecular mass of alpha-crystallin on SDS PAGE immunoblots. Specific conditions for reactivity

Images

Western Blot of mouse Anti-Alpha A Crystallin Antibody. Lane 1: AlphaA Crystallin Lane2: AlphaB Crystallin. Load: 20ug per lane. Primary antibody: AlphaA-Crystallin 1:1000 for overnight at 4C . Secondary antibody: Goat anti-Mouse IgG HRP antibody at 1:40,000 for 45 min at RT. Block: 5% Blotto overnight at 4C. Predicted/Observed size: 19.9 kDa, 20 kDa for Alpha A.