0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:200, IHC: 1:100, IF Microscopy: 1:100, WB: 1:1000
Application Notes:
Anti-EC SOD Antibody has been tested by WB, IHC and IF and is recommended for use in ELISA. Expect a band approximately ~35kDa in specific lysates. Specific conditions for reactivity should be optimized by the end user.
Immunocytochemistry of Mouse anti-SOD3 antibody. Tissue: human cartilage, (Left) Control, )Middle) Young cartilage, (Right) Cartilage sample with osteo-arthritis. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: SOD3 antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000 for 45 min at RT. Localization: SOD3 is extracellular. Staining: EC SOD as precipitated brown signal.
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