GRP78 Antibody, IgG2b, Clone: [1H11-1H7], Mouse, Monoclonal

Product Overview

• Article Name: GRP78 Antibody, IgG2b, Clone: [1H11-1H7], Mouse, Monoclonal
• Biozol Catalog Number: ROC-200-301-F36
• Supplier Catalog Number: 200-301-F36
• Alternative Catalog Number: ROC-200-301-F36
• Manufacturer: Rockland Immunochemicals
• Host: Mouse
• Category: Antikörper
• Application: IF, WB
• Species Reactivity: Bovine, Hamster, Human, Monkey, Mouse, Parasite, Rabbit, Rat, Xenopus
• Immunogen: Grp78 Antibody was produced in mice by repeated immunizations with a his-tagged human GRP78.
• Conjugation: Unconjugated
• Alternative Names: BiP, Grp78, HspA5, MIF2, immunoglobulin heavy chain binding protein, 78 kDa glucose-regulated protein, Heat shock 70 kDa protein 5

Product Properties

• Clonality: Monoclonal
• Concentration: 1mg/mL by UV absorbance at 280 nm
• Clone Designation: [1H11-1H7]
• Isotype: IgG2b
• NCBI: 3309
• UniProt: P11021
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Human
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: IF Microscopy: 1:100, WB: 1:1000
• Application Notes: Anti-GRP78 Antibody has been tested by western blot and immunocytochemistry/immunofluorescence. Expect a band approximately ~78kDa corresponding to specific lysates. Specific conditions for reactivity should be optimized by the end user.

Images

Western Blot of mouse anti-Grp78 antibody. Lane 1: molecular weight. Lane 2: HeLa cell lysates. Load: 35 µg per lane. Primary antibody: Grp78 antibody at 1:1000 for overnight at 4C. Secondary antibody: IRDye800(TM) mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 72.4 kDa, ~75 kDa for Grp78. Other band(s): none.