HDEL Antibody was produced in mice by repeated immunizations raised against a synthetic HDEL synthetic peptide corresponding to the C-terminus of yeast Bip.
Conjugation:
Unconjugated
Alternative Names:
H-D-E-L, endoplasmic reticulum, luminal ER protein retention
Clonality:
Monoclonal
Concentration:
1.0 mg/mL by UV absorbance at 280 nm
Clone Designation:
[2E7]
Isotype:
IgG2b
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Yeast
Antibody Type:
Primary Antibody
Application Dilute:
IF Microscopy: 1:50-1:500, WB: 1:1000-1:2000
Application Notes:
Anti-HDEL Antibody is tested for use in WB and IF microscopy. Expect a band approximately ~78kDa corresponding to specific lysates. Specific conditions for reactivity should be optimized by the end user.
Immunohistochemistry of mouse anti-HDEL antibody. Tissue: human keratinocyte line, HaCat. Primary Antibody: HDEL antibody at 1ug/ml for 1h at RT. Secondary antibody: Peroxidase mouse secondary at 1:10,000 for 45 min at RT. Localization: membrane.
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