HO-1 Antibody, IgG1, Clone: [1F12-A6], Mouse, Monoclonal

Product Overview

• Article Name: HO-1 Antibody, IgG1, Clone: [1F12-A6], Mouse, Monoclonal
• Biozol Catalog Number: ROC-200-301-F47
• Supplier Catalog Number: 200-301-F47
• Alternative Catalog Number: ROC-200-301-F47
• Manufacturer: Rockland Immunochemicals
• Host: Mouse
• Category: Antikörper
• Application: IF, IHC, IP, WB
• Species Reactivity: Bovine, Canine, Guinea pig, Hamster, Human, Monkey, Mouse, Porcine, Rabbit, Rat
• Immunogen: HO-1 Antibody was produced in mice by repeated immunizations raised against a human HO-1 synthetic peptide corresponding to the C-terminus region.
• Conjugation: Unconjugated
• Alternative Names: Hsp32, HMOX1, Heme oxygenase 1, HO, HO1

Product Properties

• Clonality: Monoclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Clone Designation: [1F12-A6]
• Isotype: IgG1
• NCBI: 09601
• UniProt: P09601
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Human
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:200, IHC: User Optimized, IF Microscopy: User Optimized, IP: User Optimized, WB: 1:500-1000
• Application Notes: Anti-HO-1 Antibody has been tested for WB, IP, IF microscopy, and IHC. Expect a band approximately 32kDa protein, corresponding to the molecular mass of HO-1 on SDS Page immunoblots. Specific conditions for reactivity should be optimized by the end user.

Images

Western Blot of mouse anti-HO-1 antibody. Lane 1: HeLa cell lysates. Lane 2: none. Load: 35 µg per lane. Primary antibody: HO-1 antibody at 1:1000 for overnight at 4C. Secondary antibody: IRDye800(TM) mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 32.8 kDa, ~35 kDa for HO-1. Other band(s): none.