Hsp40 Antibody was produced in mice by repeated immunizations raised against recombinant Protein Hsp40 (Hdj1).
Conjugation:
Unconjugated
Alternative Names:
DNAJ1, NDAJB1, HDJ1, Hsp40, HSPF1, DnaJ homolog subfamily B member 1, Dna J protein homolog 1, Heat shock 40 kDa protein 1, HSP40, heat shock protein 40, Human DnaJ protein 1, hDj-1
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:200, IHC: User Optimized, IF Microscopy: 10ug/ml, IP: User Optimized, WB: 1:2000
Application Notes:
Anti-Hsp40 Antibody is tested for use in WB, IP, IHC, IF, and ELISA. Expect a band approximately ~40kDa protein corresponding to the molecular mass of Hsp40 on SDS PAGE immunoblots. Specific conditions for reactivity should be optimized by the end user.
Immunofluorescence of mouse anti-Hsp40 HDJ1 antibody. Tissue: human keatinocyte line HaCat. Antigen retrieval: not required. Primary Antibody: Hsp40 HDJ1 antibody at 10ug/ml for 1h at RT. Secondary antibody: Fluorescein mouse secondary at 1:10,000 for 45 min at RT. Localization: nuclear and cytoplasmic. Staining: Hsp40 HDJ1 as green fluorescent signal.
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