NR2B Antibody, IgG2b, Clone: [S59-36], Mouse, Monoclonal

Product Overview

• Article Name: NR2B Antibody, IgG2b, Clone: [S59-36], Mouse, Monoclonal
• Biozol Catalog Number: ROC-200-301-G23
• Supplier Catalog Number: 200-301-G23
• Alternative Catalog Number: ROC-200-301-G23
• Manufacturer: Rockland Immunochemicals
• Host: Mouse
• Category: Antikörper
• Application: IF, IHC, IP, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: NR2B Antibody was produced in mice by repeated immunizations raised against a fusion protein corresponding near the extracellular N-terminus region of rat NR2B.
• Conjugation: Unconjugated
• Alternative Names: NMDAR2B, hNR3, MGC142180, glutamate receptor subunit epsilon-2, NR3, NR2B, N-methyl D-aspartate receptor subtype 2B

Product Properties

• Clonality: Monoclonal
• Concentration: 1mg/mL by UV absorbance at 280 nm
• Clone Designation: [S59-36]
• Isotype: IgG2b
• NCBI: 24410
• UniProt: Q00960
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Rat
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: IHC: 0.1-1.0ug/mL, IF Microscopy: 1.0-10ug/mL, IP: User Optimized, WB: 1-10ug/mL
• Application Notes: Anti-NR2B Antibody is tested for use in WB, IHC, IF, and IP. Expect a band approximately ~166kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Images

Western Blot of mouse anti-NR2B total antibody. Lane 1: Rat Brain Membrane Lysate. Primary antibody: NR2B total antibody at 1:1000 for overnight at 4C. Secondary antibody: Goat anti-mouse IgG:HRP secondary antibody at 1:10,000 for 45 min at RT. Block: 5% Blotto overnight 4C. Predicted/Observed size: 166kDa/166kD. Other band(s): none.