0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Rat
Antibody Type:
Primary Antibody
Application Dilute:
IHC: 0.1-1.0ug/mL, IF Microscopy: 1.0-10ug/mL, IP: User Optimized, WB: 1-10ug/mL
Application Notes:
Anti-SHANK Antibody is tested for use in WB, IHC and IP. Expect a band approximately ~160kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.
Western Blot of mouse anti-SHANK antibody. Lane 1: Rat Brain Membrane lysate. Primary antibody: SHANK antibody at 1:1000 for overnight at 4C. Secondary antibody: Goat anti-mouse IgG HRP secondary antibody at 1:10,000 for 45 min at RT. Block: 5% Blotto overnight 4C. Predicted/Observed size:158.6 kDa/160kD. Other band(s): none
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