SHANK3 Antibody, IgG2b, Clone: [S69], Mouse, Monoclonal

Catalog Number: ROC-200-301-G45
Article Name: SHANK3 Antibody, IgG2b, Clone: [S69], Mouse, Monoclonal
Biozol Catalog Number: ROC-200-301-G45
Supplier Catalog Number: 200-301-G45
Alternative Catalog Number: ROC-200-301-G45
Manufacturer: Rockland Immunochemicals
Host: Mouse
Category: Antikörper
Application: IF, IHC, IP, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: SHANK3 Antibody was produced in mice by repeated immunizations raised against a synthetic peptide corresponding to an internal region of rat Shank3.
Conjugation: Unconjugated
Alternative Names: Shank3, Prosap2, SH3 and multiple ankyrin repeat domains protein 3, Proline-rich synapse-associated protein 2
Clonality: Monoclonal
Concentration: 1.0 mg/mL by UV absorbance at 280 nm
Clone Designation: [S69]
Isotype: IgG2b
NCBI: 59312
UniProt: Q9JLU4
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form: Liquid (sterile filtered)
Target: Rat
Antibody Type: Primary Antibody
Application Dilute: IHC: 0.1-1.0ug/mL, IF Microscopy: 1.0-10ug/mL, IP: User Optimized, WB: 1-10ug/mL
Application Notes: Anti-SHANK3 Antibody is tested for use in WB, IHC, IF, and IP. Expect a band approximately ~190kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.
Western Blot of mouse anti-Shank3 antibody. Lane 1: Rat Brain Membrane lysate. Primary antibody: Shank3 antibody at 1:1000 for overnight at 4C. Secondary antibody: Goat anti-mouse IgG HRP secondary antibody at 1:10,000 for 45 min at RT. Block: 5% Blotto overnight 4C. Predicted/Observed size: 193.2 kDa/190kD. Other band(s): none.