Hemoglobin beta F Antibody, IgG1, Clone: [4B3.B5.F3.B7], Mouse, Monoclonal

Product Overview

• Article Name: Hemoglobin beta F Antibody, IgG1, Clone: [4B3.B5.F3.B7], Mouse, Monoclonal
• Biozol Catalog Number: ROC-200-301-GS6S
• Supplier Catalog Number: 200-301-GS6S
• Alternative Catalog Number: ROC-200-301-GS6S
• Manufacturer: Rockland Immunochemicals
• Host: Mouse
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Anti-Hemoglobin beta F Monoclonal Antibody was produced in mice by repeated immunizations with synthetic peptide corresponding to amino acid residues near the N-terminus of Hb beta-subunit conjugated to KLH.
• Alternative Names: mouse anti-HbF antibody, mouse anti-hemoglobin antibody, Gamma-1-globin, Hb F Agamma, Hemoglobin gamma-1 chain, Hemoglobin gamma-A chain, HBG1, HBG2, HbF Antibody, Sickle Cell Disease (SCD)

Product Properties

• Clonality: Monoclonal
• Concentration: 1.0mg/ml by UV absorbance at 280 nm
• Clone Designation: [4B3.B5.F3.B7]
• Isotype: IgG1
• NCBI: 3047
• UniProt: P69891
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Human
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:20,000, WB: 1ug/mL
• Application Notes: Anti-Hemoglobin beta F (MOUSE) antibody has been tested ELISA and Western Blotting. This antibody is designed for use in lateral flow. Specific conditions of reactivity should be optimized by the end user. Expect a band of approximately 16 kDa.

Images

Western Blot of Mouse Anti-Hemoglobin beta F Antibody. Lane 1: Molecular Weight Ladder. Lane 2: HbA peptide conjugated to BSA. Lane 3: HbA-2 peptide conjugated to BSA. Lane 4: HbC peptide conjugated to BSA. Lane 5: HbF peptide conjugated to BSA. Lane 6: HbS peptide conjugated to BSA. Load: 50ng per lane. Primary antibody: Anti-HbF antibody at 1µg/mL overnight at 4C. Secondary antibody: Rabbit Anti-Mouse secondary antibody at 1:40,000 for 30 min at RT. Block: MB-073 for 30 min RT. Predicted/Observed: Reactivity seen in Lane 5 specific to HbF only.