Hemoglobin beta A-2 Antibody, IgG1, Clone: [21G1.F1.B9.G9.D11], Mouse, Monoclonal

Catalog Number: ROC-200-301-GS8
Article Name: Hemoglobin beta A-2 Antibody, IgG1, Clone: [21G1.F1.B9.G9.D11], Mouse, Monoclonal
Biozol Catalog Number: ROC-200-301-GS8
Supplier Catalog Number: 200-301-GS8
Alternative Catalog Number: ROC-200-301-GS8
Manufacturer: Rockland Immunochemicals
Host: Mouse
Category: Antikörper
Application: ELISA, WB
Species Reactivity: Human
Immunogen: Anti-Hemoglobin beta A-2 Monoclonal Antibody was produced in mice by repeated immunizations with synthetic peptide corresponding to amino acid residues near the N-terminus of Hb delta-subunit conjugated to KLH.
Alternative Names: mouse anti-HbA-2 antibody, mouse anti-hemoglobin antibody, Hemoglobin Subunit Delta, Hemoglobin Delta Chain 4, Delta-Globin 4, HbD, HbA-2 Antibody, Sickle Cell Disease (SCD)
Clonality: Monoclonal
Concentration: 1.00 mg/ml by UV absorbance at 280 nm
Clone Designation: [21G1.F1.B9.G9.D11]
Isotype: IgG1
NCBI: 3045
UniProt: P02042
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form: Liquid (sterile filtered)
Target: Human
Antibody Type: Primary Antibody
Application Dilute: ELISA: 1:20,000, WB: 1ug/mL
Application Notes: Anti-Hemoglobin beta A-2 (MOUSE) antibody has been tested by ELISA and Western Blotting. This antibody is designed for use in lateral flow. Specific conditions of reactivity should be optimized by the end user. Expect a band of approximately 16 kDa.
Western Blot of Mouse Anti-Hemoglobin beta A-2 Antibody. Lane 1: Molecular Weight Ladder. Lane 2: HbA peptide conjugated to BSA. Lane 3: HbA-2 peptide conjugated to BSA. Lane 4: HbC peptide conjugated to BSA. Lane 5: HbF peptide conjugated to BSA. Lane 6: HbS peptide conjugated to BSA. Lane 7: BSA alone. Load: 50ng per lane. Primary antibody: Anti-HbA-2 antibody at 1µg/mL overnight at 4C. Secondary antibody: Rabbit Anti-Mouse secondary antibody at 1:40,000 for 30 min at RT. Block: MB-073 for 30 min RT. Predicted/Observed: Reactivity seen in Lane 3 specific to HbA-2 only.