0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
WB: 1-2- µg/mL
Application Notes:
Anti-Bif-1 Antibody is tested for use in WB, ICC/IF, IHC, IHC-P, and IP. Expect a band approximately 23kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.
Western Blot of Mouse Anti-Bif-1 antibody. Lane A: Jurkat Cell Lysate. Load: 10 µg per lane. Primary antibody: Bif-1 antibody at 2 µg/ml dilution for overnight at 4C. Secondary antibody: IRDye800(TM) mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 40.8/40 kDa for Bif-1. Other band(s): none.
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