0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
WB: 2-5 µg/mL
Application Notes:
Anti-IKKgamma antibody is tested for use in WB. Expect a band approximately 48kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.
Western Blot of Mouse Anti-IKKgamma antibody. Lane 1: Lysate from human Jurkat cells. Lane 2: Lysate from mouse NIH 3T3 cells with IKKgamma antibody. Primary antibody: IKKgamma antibody at 2 µg/mL for overnight at 4C. Secondary antibody: IRDye800(TM) mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 40 kDa for IKKgamma . Other band(s): none.
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