MITF Antibody was produced in mice prepared by repeated immunizations with a synthetic peptide (sequence PSTGLCSPDLVN) corresponding to internal amino acids with human MITF protein.
Conjugation:
Unconjugated
Alternative Names:
BHLHE32, Microphthalmia-associated transcription factor, Class E basic helix-loop-helix protein 32, bHLHe32
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
WB: 3-5 µg/mL
Application Notes:
Anti-MITF antibody is tested for WB, IHC, and IF. Expect a band approximately 52kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.
Western Blot of Mouse Anti-MITF antibody. Lane A: MITF in A375 lysate. Primary antibody: MITF antibody at 4 µg/mL for overnight at 4C. Secondary antibody: IRDye800(TM) mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 45 kDa for Livin BIRC7 KIAP . Other band(s): none.
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