SARS-CoV-2 Whole Spike Protein Antibody, Clone: [15D9.E2.C10], Mouse, Monoclonal

Product Overview

• Article Name: SARS-CoV-2 Whole Spike Protein Antibody, Clone: [15D9.E2.C10], Mouse, Monoclonal
• Biozol Catalog Number: ROC-200-301-MS9
• Supplier Catalog Number: 200-301-MS9
• Alternative Catalog Number: ROC-200-301-MS9
• Manufacturer: Rockland Immunochemicals
• Host: Mouse
• Category: Antikörper
• Application: ELISA
• Species Reactivity: Virus
• Immunogen: This protein A purified antibody was produced by repeated immunizations with a purified SARS-CoV-2 S protein with His-tag, derived from the transfected human HEK293 cells.
• Conjugation: Unconjugated
• Alternative Names: 2019-nCoV, COVID-19, S glycoprotein, SARS CoV2 antibody, Severe acute respiratory syndrome antibody, Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2), Spike glycoprotein antibody, mouse anti-SARS CoV 2 Spike Protein Antibody, sars-cov-2

Product Properties

• Clonality: Monoclonal
• Concentration: 1.017 mg/mL by UV absorbance at 280 nm
• Clone Designation: [15D9.E2.C10]
• NCBI: 908947
• UniProt: P0DTC2
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: SARS
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:145,000 - 1:95,000
• Application Notes: Anti-SARS-CoV-2 Whole Spike protein antibody has been tested for use in ELISA and shows reactivity to SARS COV-2 Whole Spike and COV-2 Spike (S2) protein. Specific conditions for reactivity should be optimized by the end user.

Images

ELISA results of Mouse Anti-SARS CoV-2 Whole Spike Protein. Each well was coated in duplicate with 0.5µg of rec SARS CoV-2 Whole Spike protein [RED line], rec SARS CoV-2 Spike (S1) [GREEN line], rec SARS CoV-2 Spike (S1) RBD protein [BLUE line], and rec SARS CoV-2 Spike (S2) glycoprotein [PURPLE line]. The working dilution for SARS-CoV-2 whole spike is 1:148,000 and SARS CoV-2 Spike (S2) glycoprotein is 1:93,800. The starting dilution of antibody was 0.55µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Rabbit Anti-Mouse IgG HRP conjugated (p/n 610-403-C46) at 1:8000 and TMB substrate (p/n TMBE-1000). SARS CoV-2 Whole Spike EC50: 7ng/mL.