SARS-CoV-2 Beta Variant Spike Protein Antibody, Clone: [25D10.E3.F9], Mouse, Monoclonal

Product Overview

• Article Name: SARS-CoV-2 Beta Variant Spike Protein Antibody, Clone: [25D10.E3.F9], Mouse, Monoclonal
• Biozol Catalog Number: ROC-200-301-MU5
• Supplier Catalog Number: 200-301-MU5
• Alternative Catalog Number: ROC-200-301-MU5
• Manufacturer: Rockland Immunochemicals
• Host: Mouse
• Category: Antikörper
• Application: ELISA
• Species Reactivity: Virus
• Immunogen: This protein A purified antibody was produced by repeated immunizations in mice with a synthetic peptide from an internal region of the SARS-CoV-2 Spike protein.
• Conjugation: Unconjugated
• Alternative Names: 2019-nCoV, COVID-19, S glycoprotein, SARS CoV2 antibody, Severe acute respiratory syndrome antibody, Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2), Spike glycoprotein antibody, mouse anti-SARS CoV 2 Spike Protein (Beta Variant) Antibody, sars-cov-2

Product Properties

• Clonality: Monoclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Clone Designation: [25D10.E3.F9]
• NCBI: 43416
• UniProt: P0DTC2
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: SARS
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: User Optimized, WB: User Optimized
• Application Notes: Anti-SARS-CoV-2 Beta Variant Spike Protein Antibody has been tested for use in ELISA and shows reactivity to SARS Coronavirus 2 Spike (S2) Beta Variant Protein. Specific conditions for reactivity should be optimized by the end user.

Images

ELISA results of Mouse Anti-SARS CoV-2 Beta B.1.351 Variant Spike Protein Antibody. Each well was coated in duplicate with 10µg/mL of SARS CoV-2 Beta (B.1.351 variant) [GREEN line], SARS CoV-2 (B.1.1.7 variant) [RED line], SARS CoV-2 (P.1 variant) [BLUE line], SARS CoV-2 (B.1.525 variant) [PURPLE line]. Additional assays were run against peptides representing other SARS CoV-2 variants (B.1.429/CAL.20, B.1.526, B.1.1.7, B.1.617.2) with similar negative results. The starting dilution of antibody was 5 µg/mL and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Rabbit Anti-Mouse IgG HRP conjugated (p/n 610-403-C46) at 1:8000 and TMB substrate (p/n TMBE-1000).