GATA4 protein A purified antibody was prepared in mice by repeated immunizations with a recombinant protein corresponding to the first 100 amino acids of human GATA4 protein.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:2000-1:10,000, WB: 1:500-1:1000
Application Notes:
Anti-GATA4 antibody has been tested by ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 44 kDa in size corresponding to GATA4 by western blotting in the appropriate cell lysat
Western Blot of Mouse anti-GATA4 Antibody. Lane 1: CCRF-CEM Lysate. Load: 25 µg per lane. Primary antibody: GATA-4 antibody at 1:500 overnight at 4C. Secondary antibody: anti-mouse IgG HRP antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 44.5 kDa, 44.5 kDa for GATA4.
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