Anti-Catenin gamma is tested for Immunohistochemistry -F, immunocytochemistry, and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~81.8 kDa corresponding to the appropriate cell lysate or
Western blot analysis of Catenin gamma (Plakoglobin) using anti-Catenin gamma (Plakoglobin) antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 µg of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: human CACO-2 whole cell lysates, Lane 6: human K562 whole cell lysates, Lane 7: human 293T whole cell lysates, Lane 8: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with affinity purified mouse anti-Catenin gamma (Plakoglobin) antigen monoclonal antibody at 1 µg/mL overnight at 4C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at 1:10,000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Catenin gamma (Plakoglobin) at approximately 82 kDa. The expected band size for Catenin gamma (Plakoglobin) is at 82 kDa.
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