This antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to the FLAG(TM) epitope tag peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide.
Conjugation:
FITC
Alternative Names:
mouse anti-FLAG(TM) tag FITC conjugation, Enterokinase Cleavage Site (ECS), Fluorescein conjugated mouse anti-DYKDDDDK, Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys
Clonality:
Monoclonal
Concentration:
1.0 mg/mL by UV absorbance at 280 nm
Clone Designation:
[29E4.G7]
Isotype:
IgG2a
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Lyophilized
Antibody Type:
Primary Antibody
Application Dilute:
IF Microscopy: 1:500 - 1:2,500
Application Notes:
Anti-FLAG Fluorescein has been tested in ELISA and western blot. This antibody is optimally suited for monitoring the expression of FLAG(TM) tagged fusion proteins. As such, this antibody can be used to identify fusion proteins containing the FLAG(TM) epitope.
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