DYKDDDDK Tag (Anti-FLAG) Antibody Peroxidase Conjugated, IgG2a, Clone: [29E4.G7], HRP, Mouse, Monoclonal

Product Overview

• Article Name: DYKDDDDK Tag (Anti-FLAG) Antibody Peroxidase Conjugated, IgG2a, Clone: [29E4.G7], HRP, Mouse, Monoclonal
• Biozol Catalog Number: ROC-200-303-383
• Supplier Catalog Number: 200-303-383
• Alternative Catalog Number: ROC-200-303-383
• Manufacturer: Rockland Immunochemicals
• Host: Mouse
• Category: Antikörper
• Application: ELISA, WB
• Immunogen: This antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to the FLAG(TM) epitope tag peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide.
• Conjugation: HRP
• Alternative Names: mouse anti-FLAG(TM) tag HRP conjugation, Enterokinase Cleavage Site (ECS), peroxidase conjugated mouse anti-DYKDDDDK, Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys

Product Properties

• Clonality: Monoclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Clone Designation: [29E4.G7]
• Isotype: IgG2a
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:10,000-1:50,000, IHC: 1:500-1:2,500, WB: 1:10,000
• Application Notes: Anti-FLAG Peroxidase has been tested by ELISA and western blot. This antibody is optimally suited for monitoring the expression of FLAG(TM) tagged fusion proteins. As such, this antibody can be used to identify fusion proteins containing the FLAG(TM) epitope.

Images

Western Blot of Mouse antibody for the detection of FLAG(TM) conjugated proteins Monoclonal Antibody Peroxidase Conjugated. Lane 1: FLAG(TM) Positive Control Lysates. Load: 10 µg per lane. Primary antibody: FLAG(TM) Monoclonal Antibody Peroxidase Conjugated at 1:1,000 for overnight at 4C. Secondary antibody: none. Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT. Predicted/Observed size: ~60 kDa, ~60 kDa.