Blue MW Ladder Antibody Peroxidase Conjugated, IgG1, HRP, Mouse, Monoclonal

Product Overview

• Article Name: Blue MW Ladder Antibody Peroxidase Conjugated, IgG1, HRP, Mouse, Monoclonal
• Biozol Catalog Number: ROC-200-303-V07
• Supplier Catalog Number: 200-303-V07
• Alternative Catalog Number: ROC-200-303-V07
• Manufacturer: Rockland Immunochemicals
• Host: Mouse
• Category: Antikörper
• Application: WB
• Immunogen: Anti-Blue Molecular Weight Ladder Antibody was produced in mice by repeated immunizations with the blue color used in many prestained MW markers.
• Conjugation: HRP
• Alternative Names: Molecular Weight Ladder HRP, HRP Protein ladder, HRP Protein marker, HRP Protein standard, HRP Protein molecular weight standard, HRP Molecular weight marker, Protein molecular weight ladder, Protein marker, Mw marker, Mw standard, Western blot standard, molecular weight marker, protein standard, gel electrophoresis marker

Product Properties

• Clonality: Monoclonal
• Concentration: neat by UV absorbance at 280 nm
• Isotype: IgG1
• Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB: 1:1,000 - 1:3,000
• Application Notes: Anti-Blue Ladder MW Antibody Peroxidase Conjugated is tested by SDS and western blot. Anti-Blue Ladder MW HRP Antibody is designed for Western Blots specifically, and will detect the Molecular Weight Ladder. Specific conditions for reactivity should be o

Images

SDS and WB of Mouse anti-Blue Ladder MW- Peroxidase Conjugated antibody. Figure A contains 4 µl of each ladder. Lane 1: Page Ruler Plus. Lane 2: Color Plus broad range. Lane 3: Page Ruler. Lane 4: Spectra Multicolor. Lane 5: Precision Plus. Figure B contains 1 µl of each ladder. Primary Antibody: Mouse anti-Blue Ladder MW- Peroxidase Conjugated antibody at 1:1,000. Secondary Antibody: None. Block: PBS-Tween containing 10% milk overnight at 4C. Predicted/Observed size: Antibody binds only blue bands. Other band(s): none.

SDS and WB of Mouse anti-Blue Ladder MW- Peroxidase Conjugated antibody. Lane 1: 1 µl Precision Plus protein MW marker. Lane 2: 0.5 µl Precision Plus protein MW marker. Lane 3: 0.25 µl Precision Plus protein MW marker. Primary Antibody: Mouse anti-Blue Ladder MW- Peroxidase Conjugated antibody at 1:2,000. Secondary Antibody: None. Block: PBS-Tween containing 10% milk overnight at 4C. Predicted/Observed size: Antibody binds only blue bands. Other band(s): none.

Mouse anti-Blue Ladder MW Peroxidase Conjugated antibody. Lanes 1-8: Protein extracts of Mouse, Human, Rat, Hamster, Monkey, Yeast, E. Coli, and Chicken, respectively. Lane 9: Blue MW Ladder. Primary antibody: Mouse anti-Blue Ladder MW- Peroxidase Conjugated antibody at 1:1,000. Secondary antibody: None. Stained with Ponceau S and chemiluminescent detection on x-ray film. Predicted/Observed size: Antibody binds only blue bands.

Western Blot of Mouse anti-GAPDH with Blue MW Ladder Peroxidase Conjugated. M: Opal Pre-stained Ladder (MB-210-0500). Lane 1: GAPDH [50 ng]. Primary antibody: Mouse anti-GAPDH at 1:1,000 with Blue MW Ladder Peroxidase Conjugated at 1:2,000 overnight at 4C. Secondary antibody: Rabbit anti-Mouse IgG HRP secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 36 kDa, 36 kDa for GAPDH.