Pdcd4 phospho S457 Antibody Biotin Conjugated, IgG1, Clone: [9G6], Mouse, Monoclonal

Product Overview

• Article Name: Pdcd4 phospho S457 Antibody Biotin Conjugated, IgG1, Clone: [9G6], Mouse, Monoclonal
• Biozol Catalog Number: ROC-200-306-964
• Supplier Catalog Number: 200-306-964
• Alternative Catalog Number: ROC-200-306-964
• Manufacturer: Rockland Immunochemicals
• Host: Mouse
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human, Mouse, Rat, Xenopus
• Immunogen: This monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Ser457 of the human Pdcd4 protein.
• Conjugation: Biotin
• Alternative Names: mouse anti-Pdcd4 pS457 Antibody biotin conjugation, biotin conjugated mouse anti-Pdcd4 pS457 Antibody, Death up-regulated gene protein antibody, Dug antibody, H731 antibody, Ma3 antibody, Neoplastic transformation inhibitor antibody, Neoplastic transformation inhibitor protein antibody, Nuclear antigen H731 antibody

Product Properties

• Clonality: Monoclonal
• Concentration: 0.5 mg/mL by UV absorbance at 280 nm
• Clone Designation: [9G6]
• Isotype: IgG1
• NCBI: 27250
• UniProt: Q53EL6
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Human
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:20,000 - 1:100,000, IP: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Application Notes: Anti-Pdcd4 pS457 (MOUSE) Monoclonal Antibody Biotin Conjugated has been tested by ELISA and western blotting and is suitable for immunohistochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 62

Images

Western Blot of Mouse anti-Pdcd4 phospho S457 Biotin Conjugated antibody. Lane 1: Pdcd4 recombinant protein. Lane 2: none. Load: 100 ng per lane. Primary antibody: Pdcd4 phospho S457 Biotin Conjugated antibody at 1:1,000 for overnight at 4C. Secondary antibody: HRP Streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 50 kDa, 50 kDa for Pdcd4 phospho S457. Other band(s): Pdcd4 phospho S457 splice variants and isoforms

ELISA of Mouse anti-Pdcd4 phospho S457 Biotin Conjugated antibody. Antigen: BSA conjugates of Pdcd4 phospho S457 and Pdcd4 non-phospho S457. Coating amount: 0.1 µg per well. Primary antibody: Pdcd4 phospho S457 Biotin Conjugated antibody at 5 µg/mL. Dilution series: 3-fold. Mid-point concentration: 5 ng/mL Pdcd4 phospho S457 Biotin Conjugated antibody. Secondary antibody: Peroxidase streptavidin secondary antibody at 1:10,000. Substrate: TMB (p/n TMBE-0100)