Anti-ERK2 Monoclonal Antibody was produced in mice by repeated immunizations with synthetic peptide corresponding to amino acid residues near an internal region of ERK2 conjugated to KLH.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Lyophilized
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:40,000, WB: 1µg/ml
Application Notes:
Anti-ERK 2 (MOUSE) DyLight(TM)680 conjugated antibody is suitable for use in ELISA and Western Blotting. Specific conditions of reactivity should be optimized by the end user. Expect a band of approximately 41 kDa.
Western Blot Results of Monoclonal Anti-ERK2 (MOUSE) Antibody DyLight(TM)680 Conjugated.Lane 1: Opal Prestained Molecular Weight Marker (p/n MB-210-0500). Lane 2: human rERK1 Reduced [0.05µg]. Lane 3: ERK2 Reduced [0.05µg]. Primary Antibody: Anti-ERK2 (MOUSE) Antibody DyLight(TM)680 Conjugated at 1.0µg/mL overnight at 2-8C. Secondary Antibody: Rabbit Anti-Mouse Antibody Peroxidase Conjugated (p/n 610-403-C46) at 1:40,000 for 30mins at RT. Blocking buffer: Blocking Buffer for Fluorescent Western Blotting (p/n MB-070) for 1hr at RT. Expected Molecular Weight: ~41kDa.
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