Dextranase Antibody, Rabbit, Polyclonal

Product Overview

• Article Name: Dextranase Antibody, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-200-401-089-0100
• Supplier Catalog Number: 200-401-089-0100
• Alternative Catalog Number: ROC-200-401-089-0100
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: WB
• Species Reactivity: Algae
• Immunogen: Dextranase [Penicillium species]
• Conjugation: Unconjugated
• Alternative Names: rabbit anti-Dextranase Antibody, dextranase, Alpha-1,6-glucan-6-glucanohydrolase, DEX

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/ml by UV absorbance at 280 nm
• NCBI: 47720
• UniProt: P48845
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Penicillium
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:5,000 - 1:20,000, IP: 1:100, WB: 1:500 - 1:5,000
• Application Notes: DEXTRANASE Antibody has been tested by western blot and is suitable to be assayed against 1.0 µg of Dextranase [Penicillium species] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code 611-1302 and (ABTS (

Images

Both the antiserum and IgG fractions of anti-Dextranase (Penicillium) are shown to detect under reducing conditions of SDS-PAGE the 41,000 dalton enzyme in cellular extracts. Approximately 10 ug of total protein is loaded per lane. A 1:2,000 dilution of the primary antibody is used followed by detection using HRP Goat-a-Rabbit IgG [H&L] (611-1302) diluted 1:4,000 and color development using 4-CN substrate until sufficient color develops. Other detection systems will yield similar results.